other:
Cell Culture:Article Title: Small molecule Bcl-2 functional converters as cancer therapeutics
Article Snippet: Cell Culture Cell lines were obtained from ATCC and maintained according to manufacturer's instructions. .. The human lung cancer cell line NCI-H460, H69AR (ATCC) were cultured in RPMI medium (Corning) containing 10% FBS. .. The human breast cancer cell line MDA-MB-231 was cultured in DMEM medium (Corning) containing 10% FBS.
Article Title: Small molecule BCL-2 functional converters as cancer therapeutics
Article Snippet: Cell Culture Cell lines were obtained from ATCC and maintained according to manufacturer's instructions. .. The human lung cancer cell line NCI-H460, H69AR (ATCC) were cultured in RPMI medium (Corning) containing 10% FBS. .. The human breast cancer cell line MDA-MB-231 was cultured in DMEM medium (Corning) containing 10% FBS.
Modification:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
Plasmid Preparation:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
Derivative Assay:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
Clone Assay:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
Selection:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
Reporter Assay:Article Title: Distinct ATRX functions cooperate with 9-1-1 and CST complexes to safeguard replication and telomere integrity
Article Snippet: .. The human chronic myelogenous leukemia-derived cell line eHAP (haploid purchased from Horizon Discovery albeit stable diploid cells used throughout) and the human lung cancer cell line NCI-H460 (purchased from ATCC) were further modified by a lentiviral integration of doxycycline-inducible Cas9 nuclease (iCas9) with an Edit-R inducible lentiviral Cas9 vector (Horizon Discovery) . eHAP iCas9 and NCI-H460 iCas9 (parental WT cell lines) were derived as single clones from the pool of transduced cells after blasticidin selection (8 μg/mL for eHAP, 2.5 μg/mL for NCI-H460) and were chosen based on a high cutting efficiency and negligible leakiness using a BFP/GFP reporter assay (Addgene #67980). eHAP iCas9 parental cell line (and where indicated, ATRX KO line) was used for the generation of ATRX , STN1 , CTC1 and FAM111A full knockouts. eHAP cell lines were grown in Iscove′s modified Dulbecco′s medium (IMDM) with 10% Tet-free FBS and 1% penicillin/streptomycin. ..
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